FastQCFastQC Report
Fri 25 Nov 2016
G101M7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101M7.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences10718782
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1659941.5486274466632497TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA177200.042.191921
ATCGGAA178750.041.8014452
CGTATGC178150.041.62414644
TCGTATG178500.041.5178843
ACACGTC181250.041.42215713
ACGTCTG181450.041.38842815
CTCGTAT180300.041.10396642
CGTCTGA182850.041.04747416
TCTCGTA183600.040.3801741
CACGTCT186500.040.32669414
CACACGT186950.040.2180512
ATCTCGT186500.039.7554240
CGGAAGA191850.039.4367874
GCACACG193250.039.0207611
TCGGAAG194900.038.437463
CATCTCG194150.038.2805139
ACCAGAT198200.037.76664732
AGTCACC205050.036.4923928
GATCATC204600.036.3488636
AGAGCAC209350.036.198518
Produced by FastQC (version 0.11.2)