FastQCFastQC Report
Fri 25 Nov 2016
G101M8.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101M8.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences18840210
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC5583722.9637249266329833TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA598050.043.0291751
ATCGGAA603300.042.6073152
ACACGTC605450.042.5810913
CGTATGC598450.042.47232444
TCGTATG599700.042.41681343
CGTCTGA607400.042.37161616
ACGTCTG608200.042.33758515
AATCTCG606850.042.13712739
CTCGTAT603500.042.13548342
CACACGT615950.041.93378412
CACGTCT614950.041.9265214
TCTCGTA608700.041.83056341
CGGAAGA620950.041.746024
ATCTCGT614350.041.4278940
GCACACG625000.041.38993511
TCGGAAG628900.040.9157073
TTGAATC624950.040.88776436
GAATCTC641300.039.8795338
TGAATCT644750.039.67307337
CACTTGA647300.039.6660433
Produced by FastQC (version 0.11.2)