FastQCFastQC Report
Fri 25 Nov 2016
G101M9.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101M9.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences11613396
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC167200.14397166857997437TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25850.033.023881
TCGTATG26600.031.75492543
CGTATGC28800.029.40558244
ACACGTC29650.029.30535713
ACGTCTG30150.028.74553715
AGTCACG30150.028.38685828
CGTCTGA31000.028.0283116
CTCGTAT30850.027.38037942
GTCACGA31400.027.11701829
CACGTCT33450.025.90965514
CACACGT34650.024.9496212
ACGATCA34650.024.82852732
TCACGAT36650.023.77288230
CGATCAG36500.023.57009533
TCTCGTA37450.022.67491141
ATCGGAA37800.022.5255832
GATCTCG38450.022.43166739
CACGATC39900.021.6714431
CGGAAGA43200.020.4207944
ATCTCGT42950.019.9271540
Produced by FastQC (version 0.11.2)