FastQCFastQC Report
Sat 26 Nov 2016
G114-M2_TGACCA_AC6GPWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG114-M2_TGACCA_AC6GPWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21797760
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG15050.011.69506243
GATCGGA15200.011.3058521
CGGGCTA10950.010.8650541
GTCGGTT19600.010.5663261
CGTATGC17350.010.01822144
GCCGGAT20300.09.7678441
CCCGTAT13750.09.6139271
ATCGGAA19600.08.8665222
CGCGTAT4602.210254E-88.6211841
CTCGTAT20550.08.56523242
GTCGGAT20250.08.4863671
TACCGAA19550.08.4392121
CTCCGAT25950.08.1505351
ACGTCTG24200.07.999255715
CGGGCAA27600.07.9825781
GCGGGTT15000.07.93148951
GTCCAAT42450.07.837031
CTACCGA19950.07.828935620
ACTACCG19500.07.78398119
CGCCTAT6901.2914825E-107.6632751
Produced by FastQC (version 0.11.2)