FastQCFastQC Report
Sat 26 Nov 2016
G114-M3_ACAGTG_AC6GPWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG114-M3_ACAGTG_AC6GPWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23310599
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16600.014.6004641
TCGTATG20400.012.61827743
CGTATGC21150.011.651144
CTCGTAT22300.010.95143142
GTCGGAT17800.010.8929321
GTCGCAT11800.010.2698181
ACACGTC25100.09.90364513
ATCGGAA24050.09.7871712
ACGTCTG28200.09.12694115
CGTCTGA29250.08.94972216
GTCGACA16350.08.6247061
ACGTTCG6950.08.5460896
TCGGATT22200.08.5218512
TCGCATT14800.08.32366852
GTCGGAA26950.08.0121561
GTCGATT12850.07.88745361
GGCGGAT28700.07.6771531
CTCCGAT31650.07.6577481
GGCGTAT24350.07.5103681
CGTGCGA6207.852577E-97.451841438
Produced by FastQC (version 0.11.2)