FastQCFastQC Report
Sat 26 Nov 2016
G122-M10_AGTTCC-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M10_AGTTCC-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18311481
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGGT26050.09.79637432
TAGCGTA7950.09.68507637
AACGCGT11850.09.2821588
CACGGAC27300.09.268023517
CCGGACA24500.09.24876912
CGCAATC5950.09.24330939
TCTAACG12900.09.2090175
CGTACGG21750.09.10306635
CTAATCG13300.08.601663
CTAACGC14350.08.5848666
CGGTAAT10050.08.31896819
ACGGACA28050.08.31428418
TCGACCG15250.08.22354920
GCGTACG25450.08.12552934
TCACCCG27450.08.0948653
ATCGATC19300.08.09279256
TCGATCG17600.07.99955467
GGCGTAC26050.07.938376433
CGATCGT17600.07.8745418
GTACGGA26550.07.871566336
Produced by FastQC (version 0.11.2)