FastQCFastQC Report
Sat 26 Nov 2016
G122-M8_CTTGTA-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M8_CTTGTA-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21036503
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC313640.14909322143514062TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA48500.034.7818451
TCGTATG53300.031.57634243
ATCGGAA55000.030.7806192
CGTATGC56150.029.8562244
ACACGTC58950.029.2520713
CTCGTAT55600.028.9233442
AATCTCG53900.028.15889439
CGTCTGA61400.028.15468216
ACGTCTG61800.027.83036215
CACGTCT69200.024.8230314
CACACGT71250.024.2976212
CGGAAGA72050.024.2920044
TCTCGTA63350.024.20277641
GCACACG74350.023.19903211
ATCTCGT69450.022.20335640
TCGGAAG80950.021.6212863
GTAATCT73650.020.69695737
TAATCTC81050.018.67199538
TGTAATC86750.017.72350336
AGTCACC101750.017.20707728
Produced by FastQC (version 0.11.2)