FastQCFastQC Report
Fri 25 Nov 2016
G145-M11_GGCTAC_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M11_GGCTAC_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18708667
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG635840.3398638716483649Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT95100.039.15000511
CGTCGTG95600.039.01294312
CGTGTAG98500.037.79408615
TCGTGTA96550.037.78503414
GATCTCG100400.037.09353334
AGCGTCG100800.036.9342910
GATCGGA110200.036.5235631
GTCGTGT102250.036.08802413
GTCGCCG96650.035.7321244
TCTCGGT103400.035.65193636
GTAGGGA105100.035.45509318
GTAGATC106600.035.30803731
GAGCGTC105450.035.266239
ATCTCGG106650.035.1028435
AGAGCGT106400.035.0340848
GTGGTCG100550.034.42973741
ATCGGAA117550.033.9403422
GGTCGCC102150.033.9362443
TGTAGGG112900.033.82544317
GTGTAGG114900.033.35474416
Produced by FastQC (version 0.11.2)