FastQCFastQC Report
Fri 25 Nov 2016
G145-M12_CTTGTA_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M12_CTTGTA_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19917978
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1606660.806638103526372TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA199850.041.1835981
CGTATGC198250.040.8271744
TCGTATG199900.040.51218843
ATCGGAA205700.039.9128232
ACACGTC208000.039.66090813
CGTCTGA209250.039.47911516
ACGTCTG210300.039.3552115
AATCTCG188500.039.06864539
CTCGTAT199150.039.00797342
CACGTCT218750.037.8041514
TCTCGTA195550.037.7618741
CACACGT220150.037.57193812
CGGAAGA222400.037.024554
TCGGAAG227800.036.1661953
GCACACG230450.035.93010311
ATCTCGT206700.035.72303840
GTAATCT207450.035.6332837
TAATCTC211750.034.9446338
TGTAATC218150.033.9054336
TTGTAAT228100.033.46026635
Produced by FastQC (version 0.11.2)