FastQCFastQC Report
Fri 25 Nov 2016
G145-M13_AGTCAA_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M13_AGTCAA_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20028576
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT583350.2912588493560401TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA82600.037.5421181
ACACGTC90300.034.70596313
ATCGGAA89600.034.486632
CTCGTAT84000.033.86527344
ACGTCTG94150.033.21941815
CGTCTGA94750.032.8929916
AATCTCG82150.032.0075241
CACGTCT100550.031.23546814
TCTCGTA86650.030.67140443
CACACGT103350.030.51531212
CGGAAGA108250.028.7279034
TCGGAAG110600.028.2566933
AGTCAAC110850.027.9948534
GCACACG115100.027.32317411
ATCTCGT97300.027.17886442
CAGTCAA131500.023.81419833
GTCAACA130650.023.73467635
GAACTCC140100.022.74895521
AACAATC117800.022.71924438
TCTGAAC143650.022.10965218
Produced by FastQC (version 0.11.2)