FastQCFastQC Report
Fri 25 Nov 2016
G145-M13_AGTCAA_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M13_AGTCAA_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20028576
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG476880.23809980300147152Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT69350.037.31531511
CGTCGTG71000.036.69478612
CGTGTAG74050.035.47744415
TCGTGTA73900.035.1945514
GATCTCG75550.034.4450534
GATCGGA81750.034.1598851
AGCGTCG78700.033.18812610
GTAGATC79500.033.0106631
GTCGTGT79800.032.75756513
TCTCGGT80000.032.25012636
GTCGCCG75850.032.24841344
ATCGGAA87800.031.4802132
ATCTCGG83300.031.44936235
GAGCGTC83500.031.3342639
GTAGGGA84000.031.21479618
GGTCGCC78050.031.19726643
AGAGCGT84650.031.090318
TGGTCGC77850.031.04928642
GTGGTCG80400.030.5560141
TGTAGGG91550.029.07377217
Produced by FastQC (version 0.11.2)