FastQCFastQC Report
Fri 25 Nov 2016
G145-M15_ATGTCA_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M15_ATGTCA_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17902178
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT236950.13235819686297387TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA39500.032.8841251
ATCGGAA43800.029.4746932
ACACGTC45550.029.40419813
CTCGTAT43000.027.83331744
ACGTCTG49350.027.2317615
CGTCTGA49250.027.01909816
AATCTCG42400.025.73928341
CACACGT54900.024.35618412
TCTCGTA46050.023.74440443
CACGTCT56500.023.66810414
CGGAAGA60400.022.3935764
ATCTCGT51250.021.33563442
GCACACG63900.020.95980311
TCGGAAG67750.019.8018533
ACATGTC79300.016.78430632
GAATCTC72900.015.18499140
GAACTCC91950.014.9507221
TCTGAAC95400.014.57131918
ATGTCAG92350.014.53669934
CACATGT91600.014.43400331
Produced by FastQC (version 0.11.2)