FastQCFastQC Report
Fri 25 Nov 2016
G145-M16_CCGTCC_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M16_CCGTCC_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18880824
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG489240.25912004687930995Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT77950.037.96901711
CGTCGTG79400.037.35748312
CGTGTAG80600.037.09747315
TCGTGTA80000.036.47063414
GATCGGA93400.035.2476161
AGCGTCG84350.035.13836310
GATCTCG84050.034.9390734
TCTCGGT84350.034.5241936
GTCGTGT86350.034.37528613
GTAGGGA88250.033.87348618
GTAGATC89850.033.6154331
GTGGTCG82100.033.45930541
ATCTCGG89750.032.88905335
GAGCGTC90350.032.8797729
ATCGGAA100100.032.3607672
AGAGCGT92750.032.2187778
TGTAGGG95450.031.98808717
TGGTCGC85450.031.83928542
GGTCGCC85350.031.80130443
GTCGCCG85850.031.6174344
Produced by FastQC (version 0.11.2)