FastQCFastQC Report
Fri 25 Nov 2016
G145-M1_ATCACG_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M1_ATCACG_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19435498
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC558520.28737107739662754TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA79350.036.295631
TCGTATG79500.035.81036443
CGTATGC82700.034.63754344
ATCGGAA85350.033.576462
ACACGTC89350.032.83452613
CTCGTAT83650.032.77138542
ACGTCTG89650.032.65290515
ATCACGA87050.032.61604734
CGTCTGA89800.032.35344716
ACGATCT81550.031.9532237
TCACGAT85600.031.26471935
CACGTCT98800.029.71761314
CACACGT99850.029.4918512
TCTCGTA89200.029.3300941
CACGATC89000.029.20332136
CGGAAGA102300.028.5291824
ATCTCGT92850.028.2327840
CATCACG105400.027.28995933
GATCTCG96700.027.17840839
TCGGAAG107800.026.9307983
Produced by FastQC (version 0.11.2)