FastQCFastQC Report
Fri 25 Nov 2016
G145-M2_CGATGT_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M2_CGATGT_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19326534
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG295300.15279511577192267Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT45700.036.64333711
CGTGTAG50500.033.46057515
CGTCGTG50400.033.39954812
AGCGTCG52350.032.8702610
TCGTGTA50950.032.4331914
GTCGTGT54100.031.03337913
GATCTCG55750.030.4998834
TCTCGGT56500.030.16912836
GATCGGA63800.029.2166821
ATCTCGG59550.029.06843235
GTAGATC59750.028.82791331
GTCGCCG54350.028.65345244
GAGCGTC61350.028.4087289
GTAGGGA61050.028.14030818
TGTAGGG65150.026.9440717
ATCGGAA69950.026.4591852
AGAGCGT66750.026.30838
GTGGTCG59800.026.29559141
CGGTGGT63750.025.63072439
TGGTCGC61400.025.5396542
Produced by FastQC (version 0.11.2)