FastQCFastQC Report
Fri 25 Nov 2016
G145-M5_ACAGTG_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M5_ACAGTG_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19386672
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC353660.18242429644448516TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA53750.033.7814331
TCGTATG55000.032.52103843
CGTATGC56150.031.93334244
ACACGTC59850.030.75678813
CTCGTAT58550.029.49724842
ATCGGAA61800.029.3245092
ACGTCTG64850.028.45529215
CGTCTGA65200.028.10013816
CACACGT70150.026.616912
TCTCGTA64650.025.322241
CACGTCT72700.025.32171414
CGGAAGA73400.025.1395744
GCACACG79700.023.56497211
ATCTCGT70200.023.32534840
GATCTCG70650.023.27143539
AGTGATC70000.023.20089136
TCGGAAG82850.022.0862923
GAACTCC109600.017.39952921
GTCACAC112450.016.70702629
TCTGAAC111700.016.69814718
Produced by FastQC (version 0.11.2)