FastQCFastQC Report
Fri 25 Nov 2016
G145-M6_GCCAAT_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M6_GCCAAT_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17321882
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC412230.237982223871517TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA59050.036.882321
ACGCCAA61350.034.46094532
TCGTATG62350.034.29755843
TATCTCG58500.034.27383839
CGCCAAT62500.033.83614733
ATCGGAA64600.033.6371082
CTCGTAT63100.033.61126742
CGTATGC63550.033.58069244
ACACGTC65700.033.3742513
GTCACGC63950.033.19623629
ACGTCTG67850.032.48120515
AGTCACG67650.031.67274728
CGTCTGA70000.031.20078316
CACGTCT73650.029.77319514
TCACGCC72400.029.68643830
TCTCGTA68600.029.66939741
CACACGT76250.028.81425312
CGGAAGA79200.028.4082624
CACGCCA75900.028.14359331
ATCTCGT73100.027.90896840
Produced by FastQC (version 0.11.2)