FastQCFastQC Report
Fri 25 Nov 2016
G145-M7_CAGATC_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M7_CAGATC_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19643562
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC529830.2697219577589849TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA73900.036.5953871
TCGTATG76400.034.64245243
CGTATGC76400.034.5848644
CTCGTAT76100.033.6233842
ACACGTC81450.033.58912313
ATCGGAA81200.033.0426062
ACGTCTG84300.032.4296915
CGTCTGA87500.031.16828216
TCTCGTA81250.030.06141
CACGTCT91950.029.8986814
CACACGT93750.029.34636512
CGGAAGA97800.027.793944
ATCTCGT89250.027.44797140
GCACACG102700.026.7453411
TCGGAAG104850.025.9460813
CATCTCG99050.024.82191339
ACCAGAT114350.023.4523432
AGTCACC118650.023.30550628
GATCATC108050.022.8121836
GTCTGAA131450.020.88178417
Produced by FastQC (version 0.11.2)