FastQCFastQC Report
Fri 25 Nov 2016
G145-M9_GATCAG_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M9_GATCAG_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18909032
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3650371.9304901488346946TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA449300.042.4520261
ATCGGAA468900.040.547432
CGTATGC472950.039.85320744
TCGTATG474400.039.7174843
ACGATCA481650.039.3186532
CGATCAG482900.039.2186533
ACACGTC488350.039.21570613
ACGTCTG489650.039.11469315
CGTCTGA489550.039.07785816
TCACGAT486900.039.001930
CTCGTAT463000.038.8804842
TCGGAAG489900.038.8721733
AGTCACG489850.038.7981528
CACGATC489400.038.68129331
CACGTCT497750.038.47756214
CACACGT498300.038.47678812
GTCACGA494550.038.39414629
TCTCGTA438400.038.2907541
CGGAAGA500500.038.0664864
GATCTCG444400.037.6896739
Produced by FastQC (version 0.11.2)