FastQCFastQC Report
Fri 25 Nov 2016
G146-M17_GTCCGC_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M17_GTCCGC_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16394331
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1346700.821442485210284TruSeq Adapter, Index 18 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260350.1588048942039782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG176450.042.36172532
GATCGGA179300.042.121791
CGTCCGC180700.041.3724633
ATCGGAA182900.040.9564822
GTCCGCA182800.040.76511834
CTCGTAT170450.040.6950844
ACACGTC186350.040.41079313
ACGTCTG187700.040.31860415
CGCACAT169250.040.17059737
CGTCTGA188350.040.13300316
AGTCACG188500.039.859728
TCCGCAC187250.039.64354335
TCTCGTA163400.039.61139343
GTCACGT189700.039.53967329
CACGTCT191900.039.35667414
TCACGTC191200.039.27549730
CCGCACA190050.038.9083436
CACACGT193900.038.88266812
CGGAAGA196650.038.260524
TCGGAAG197950.038.0870253
Produced by FastQC (version 0.11.2)