FastQCFastQC Report
Fri 25 Nov 2016
G146-M18_GTGAAA_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M18_GTGAAA_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19117276
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT981040.5131693448376223TruSeq Adapter, Index 19 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA432740.22636070117939394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA140600.040.867831
ATCGGAA144850.039.613792
CGTGAAA146150.039.10774633
CTCGTAT127650.038.9138444
TGAAACG146850.038.78664435
ACACGTC150450.038.62190613
CGTCTGA150750.038.48517616
GAAACGA147300.038.3833636
ACGTCTG151350.038.27458615
AACGATC117750.038.26334838
ACGTGAA150350.038.16900332
AAACGAT134300.038.10421437
CACGTCT156400.037.0682214
GTCACGT155600.037.06566629
AGTCACG156500.036.8647328
CACGTGA156600.036.81502531
ACGATCT122650.036.80694639
TCTCGTA122450.036.7777443
CACACGT158950.036.62574812
TCACGTG162950.035.39378730
Produced by FastQC (version 0.11.2)