FastQCFastQC Report
Fri 25 Nov 2016
G146-M18_GTGAAA_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M18_GTGAAA_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19117276
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG691240.36157871027232125Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA386890.20237715875420748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT102850.040.64826611
CGTCGTG105300.039.99516312
GATCGGA123600.038.175661
CGTGTAG109300.038.06267515
AGCGTCG110900.038.05592710
TCGTGTA108200.037.8006614
GTCGTGT111150.037.17767713
GATCTCG111500.037.1683934
TCTCGGT109250.036.92684636
GAGCGTC115250.036.67729
GTAGATC115100.036.46578631
AGAGCGT117150.036.401768
GTCGCCG104850.036.4000744
ATCTCGG114950.035.86139735
ATCGGAA129650.035.562832
TGGTCGC108350.035.38573542
GGTCGCC109850.035.1627743
GTAGGGA119900.035.1355618
GTGGTCG110750.034.89587841
GTGTAGG121400.034.6859416
Produced by FastQC (version 0.11.2)