FastQCFastQC Report
Fri 25 Nov 2016
G146-M19_GTGGCC_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M19_GTGGCC_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18426152
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT284720.15451951118171606TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA46900.033.670321
ACACGTC53650.030.13054313
TATCTCG44700.030.12979141
ATCGGAA52400.029.9260862
ACGTCTG54750.029.76515215
CTCGTAT49450.029.40507744
CGTCTGA56100.028.77461816
AGTCACG58600.027.1746828
ACGTGGC59700.026.53791232
CACACGT63100.025.8272512
TCTCGTA52900.025.70082543
CACGTCT63250.025.69645514
GTCACGT62050.025.62900729
CGTGGCC63150.024.84829933
TCACGTG67800.023.45545830
GCACACG70250.023.32379711
CGGAAGA69650.023.0196174
ATCTCGT59500.022.85295542
GGCCTTA68950.022.4388436
GCCTTAT63700.022.3895637
Produced by FastQC (version 0.11.2)