FastQCFastQC Report
Fri 25 Nov 2016
G146-M19_GTGGCC_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M19_GTGGCC_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18426152
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA187570.10179553495488369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT32700.032.9716411
CGTCGTG35800.030.42403212
CGTGTAG38250.028.98886115
TCGTGTA37050.028.74195914
GATCGGA44700.028.7019271
GTCGTGT37700.028.30730413
AGCGTCG40000.027.1747210
GATCTCG40100.027.0988634
GTAGATC45000.025.4694531
TCTCGGT41500.025.44198436
AGAGCGT44300.024.8352178
GTAGGGA45300.024.67014318
GAGCGTC44950.024.476029
GTCGCCG41750.023.86826144
ATCTCGG45400.023.83862535
ATCGGAA54150.023.1651252
TGGTCGC42950.022.8929142
GTGTAGG49950.022.55116316
GGTCGCC45800.022.18876543
GTGGTCG45350.022.06869141
Produced by FastQC (version 0.11.2)