FastQCFastQC Report
Fri 25 Nov 2016
G146-M20_GTTTCG_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M20_GTTTCG_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18618626
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT594910.31952411525963303TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG81100.040.5421633
GATCGGA85200.039.2723121
CTCGTAT81550.037.14570244
ATCGGAA89850.037.0596582
ACGTTTC91200.036.26382432
ACACGTC92500.036.2356213
CGTCTGA93150.036.02853816
TCGGAAT83400.035.67992437
ACGTCTG95450.035.2294115
CGGAATC78950.035.1541238
GTTTCGG94550.034.7519334
AGTCACG97850.034.25699228
TTCGGAA94950.034.21066736
TCTCGTA82800.033.95655443
AATCTCG82000.033.8951441
GTCACGT99100.033.78204729
CACGTCT100650.033.38889314
CACACGT102250.032.97397612
TTTCGGA100650.032.51455735
CGGAAGA107400.031.20864
Produced by FastQC (version 0.11.2)