FastQCFastQC Report
Fri 25 Nov 2016
G146-M20_GTTTCG_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M20_GTTTCG_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18618626
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG398110.21382351200351732Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA282370.1516599560031981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT61450.038.99341211
CGTCGTG63800.037.1432812
GATCGGA77200.035.5769651
TCGTGTA65550.035.0072814
AGCGTCG70750.034.30372610
CGTGTAG69550.034.19462215
GATCTCG70800.033.95913734
GTCGTGT68950.033.95440313
TCTCGGT70650.033.6573436
GTCGCCG68050.033.0047744
GAGCGTC74000.032.916619
AGAGCGT75300.032.8453648
TGGTCGC68500.032.49796742
GGTCGCC70350.032.29973243
GTAGATC76450.032.0546831
ATCTCGG75650.031.9273735
GTGGTCG71550.031.69542541
GTAGGGA76700.031.26287318
ATCGGAA86800.030.983892
GTGTAGG80950.030.08585416
Produced by FastQC (version 0.11.2)