FastQCFastQC Report
Fri 25 Nov 2016
G146-M21_CGTACG_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M21_CGTACG_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17566538
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT535570.3048807909674633TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG71950.040.9888233
ACCGTAC73650.040.09539832
CGTACGT73450.040.03257434
GTACGTA74400.039.40319435
TACGTAA75350.038.81836
GATCGGA78100.038.7717821
ACGTAAT71900.037.50081637
CGTAATC66050.037.42315738
CACCGTA80250.037.1665831
ACACGTC83250.036.35111613
ATCGGAA83450.036.2646142
CGTCTGA84900.035.61752316
CTCGTAT75400.035.44835344
AATCTCG70900.035.01222241
ACGTCTG88100.034.34867515
TCTCGTA73650.034.0831643
GTCACCG89900.033.4215429
CACGTCT92750.032.72266814
CACACGT93650.032.3377412
CGGAAGA99600.030.6493384
Produced by FastQC (version 0.11.2)