FastQCFastQC Report
Fri 25 Nov 2016
G146-M21_CGTACG_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M21_CGTACG_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17566538
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG326420.18581919784080392Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT51750.038.3936411
CGTCGTG54950.036.15789812
GATCGGA65550.035.6212121
AGCGTCG58250.034.56373610
TCGTGTA56350.034.43653514
CGTGTAG59500.033.86908715
GTCGTGT60600.032.46051413
TCTCGGT61600.032.4591936
GAGCGTC62200.032.3690459
GATCTCG62400.032.29090534
GTAGGGA63900.032.2587618
GTAGATC63850.032.24809631
ATCGGAA71700.031.7068672
GTCGCCG58550.031.4842944
AGAGCGT66900.030.728
TGGTCGC59250.030.5911542
TGTAGGG68650.030.57193617
GTGGTCG61450.030.35383841
GTGTAGG69600.030.15558816
ATCTCGG68300.029.92002135
Produced by FastQC (version 0.11.2)