FastQCFastQC Report
Fri 25 Nov 2016
G146-M23_ACTGAT_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M23_ACTGAT_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18029917
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1374920.7625769991065405TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA178000.042.039841
ATCGGAA183250.040.5542532
ACACGTC186900.040.35007513
CTCGTAT172400.039.95383544
CGTCTGA188250.039.88413616
TATCTCG165050.039.37414641
ACGTCTG191150.039.37119315
TCTCGTA168550.038.71498543
CACACGT196700.038.39565712
CACGTCT197400.038.32634714
ATCTCGT174900.037.0492342
GATATAT186950.036.79032537
GCACACG207100.036.4675311
TCGGAAG206450.036.2845653
CGGAAGA206500.036.137324
ATATCTC182300.035.6274240
TGATATA208350.035.19488536
ATATATC186400.035.1041238
ACTGATA213700.034.7472734
TATATCT188450.034.65284739
Produced by FastQC (version 0.11.2)