FastQCFastQC Report
Fri 25 Nov 2016
G146-M23_ACTGAT_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M23_ACTGAT_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18029917
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG956770.5306569076274728Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA232000.1286750238506367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT134600.041.6037311
CGTCGTG137400.041.07653412
CGTGTAG141450.040.09675615
GATCGGA160050.039.97391
TCGTGTA138600.039.92334414
AGCGTCG141550.039.70168710
GTCGTGT142200.039.1951513
GATCTCG145750.038.99841734
TCTCGGT145550.038.67342836
GTCGCCG135200.038.5605444
GAGCGTC146500.038.4204189
GTAGGGA150250.038.41985318
ATCGGAA164400.038.381252
GTAGATC151250.038.26595731
AGAGCGT152250.037.9089168
GGTCGCC137700.037.71469543
ATCTCGG150950.037.59679435
TGGTCGC137350.037.45868342
TGTAGGG156750.037.31883217
GTGGTCG140050.037.3164141
Produced by FastQC (version 0.11.2)