FastQCFastQC Report
Fri 25 Nov 2016
G146-M24_ATTCCT_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M24_ATTCCT_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16700644
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25700.030.162111
CTCGTAT28200.025.19727344
ATCGGAA31000.024.8310222
ACACGTC33300.023.9744913
TATCTCG28050.023.92849541
CGTCTGA34650.022.7857816
ACGTCTG37550.021.26036315
TCTCGTA32400.021.11751643
CACACGT40900.019.30448212
CACGTCT41800.019.20452514
ATCTCGT39050.017.41015242
GCACACG47150.017.02543311
CGGAAGA50900.015.9871924
TCGGAAG51300.015.4766913
GTCTGAA71000.011.83292117
ACATTCC70750.011.6633532
TTATCTC59400.011.5600840
TCTGAAC76200.011.285356518
GTCGGAT9700.011.1560921
GAACTCC74850.011.0496721
Produced by FastQC (version 0.11.2)