FastQCFastQC Report
Fri 25 Nov 2016
G146-M24_ATTCCT_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M24_ATTCCT_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16700644
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT21000.030.1762511
CGTCGTG23800.026.90362412
TCGTGTA24250.026.03909114
CGTGTAG25200.025.14371515
AGCGTCG27400.024.2525610
GATCGGA29300.023.4351
GTCGTGT28300.022.62566213
GATCTCG28800.022.37895234
GTAGGGA30800.021.49860818
GTAGATC32250.020.59959831
TCTCGGT30550.020.4487436
TGTAGGG33000.020.2659617
GAGCGTC32800.020.0586859
AGAGCGT34350.019.4739728
GTGGTCG31400.019.40423641
GTGTAGG34700.019.08433716
TGGTCGC31900.018.96275542
GTCGCCG31800.018.74631544
ATCGGAA37500.018.3108222
ATCTCGG35550.017.8203535
Produced by FastQC (version 0.11.2)