FastQCFastQC Report
Fri 25 Nov 2016
G146-M25_ATCACG_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M25_ATCACG_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16972860
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC431870.25444739425176427TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA59800.038.484231
TCGTATG61350.036.21870843
CGTATGC61650.035.9329744
ATCGGAA63350.035.8625952
ACACGTC65000.035.4263513
ATCACGA63150.035.3031434
ACGTCTG66400.034.51247815
CGTCTGA68800.033.14911316
CTCGTAT63000.033.14327242
ACGATCT59150.033.00448637
TCACGAT64900.032.2827735
TCTCGTA61650.031.94737241
CACGATC62700.031.44941336
CACACGT73950.031.37670712
CACGTCT75250.030.57160814
CATCACG73550.030.34091433
CGGAAGA76950.030.1245064
CGATCTC69350.028.46780238
GCACACG82750.028.11970311
GATCTCG70900.027.44229339
Produced by FastQC (version 0.11.2)