FastQCFastQC Report
Fri 25 Nov 2016
G146-M25_ATCACG_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M25_ATCACG_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16972860
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG284620.16769124354999687Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT45700.037.74692511
CGTCGTG47300.036.37714412
GATCGGA57250.034.059121
TCGTGTA49900.032.8917314
GATCTCG52350.032.6486934
GTCGCCG47250.032.5427844
AGCGTCG53950.032.3833910
TCTCGGT51650.032.32457736
GTCGTGT53300.031.5806513
CGTGTAG53800.031.24173715
GAGCGTC56850.030.9252599
AGAGCGT57000.030.8052738
GTAGATC59050.029.72785431
ATCTCGG57200.029.72692535
GTAGGGA58200.029.29407718
GGTCGCC53550.029.00057843
TGGTCGC54100.028.29923242
GTGGTCG55850.028.23902541
ATCGGAA68650.028.051132
TGTAGGG63100.027.33367317
Produced by FastQC (version 0.11.2)