FastQCFastQC Report
Fri 25 Nov 2016
G146-M26_CGATGT_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M26_CGATGT_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18485874
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC225810.12215273132338779TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA35950.031.3326321
TCACCGA38650.028.91046330
TCGTATG39350.028.68148243
ATCGGAA40400.028.036842
CGTATGC40600.027.52592744
ACACGTC42550.026.92936713
TATCTCG39550.026.26797739
CTCGTAT42450.025.86390542
ACGTCTG44850.025.84196715
CGTCTGA44650.025.46530216
ACCGATG44200.025.33973332
CGATGTA44300.025.18768934
CACCGAT45450.024.68188531
GTCACCG46500.024.36099829
CCGATGT46100.024.15622133
TCTCGTA43350.023.9610841
CACGTCT49150.023.35796414
CACACGT51200.022.3368212
CGGAAGA55250.021.3769254
GCACACG58700.019.59528411
Produced by FastQC (version 0.11.2)