FastQCFastQC Report
Fri 25 Nov 2016
G146-M26_CGATGT_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M26_CGATGT_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18485874
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT24550.031.36991111
CGTCGTG27550.027.71449312
GATCGGA34850.025.5753651
TCGTGTA30850.025.24705514
GATCTCG32750.024.1801334
GTCGTGT32750.023.85165213
CGTGTAG34400.023.66343115
AGCGTCG33800.023.6316310
TCTCGGT34250.022.79946336
GTAGATC36250.022.27117331
ATCTCGG36900.021.63952435
GTCGCCG33200.021.20246744
GTAGGGA39900.021.17195718
GAGCGTC38300.021.0849919
AGAGCGT39600.020.3372948
TGGTCGC36550.020.2213442
GTGTAGG43250.019.38087316
ATCGGAA46200.019.149492
TGTAGGG44650.018.57523517
GTGGTCG39550.018.24190141
Produced by FastQC (version 0.11.2)