FastQCFastQC Report
Fri 25 Nov 2016
G146-M28_TGACCA_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M28_TGACCA_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17398747
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22350.028.1589641
TCGTATG25350.024.55990843
CGTATGC27000.022.73186144
ATCGGAA27900.022.7028542
ACACGTC28850.022.03144513
CGTCTGA30550.020.9487316
ACGTCTG31350.020.55451815
CTCGTAT29250.020.08375242
AATCTCG28900.019.26951239
TCTCGTA32250.017.33208741
CACACGT38950.017.16552412
CACGTCT38650.016.78662714
CGGAAGA41800.016.2582724
GCACACG47800.013.94137711
TCGGAAG48650.013.9238743
GACCAAT45500.013.39881635
ATCTCGT41900.013.28983440
ACTGACC50250.013.04895932
CGTGCGA4400.012.00588338
ACCAATC51900.011.61920236
Produced by FastQC (version 0.11.2)