FastQCFastQC Report
Fri 25 Nov 2016
G146-M30_GCCAAT_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M30_GCCAAT_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17575309
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT17150.027.96924811
CGTCGTG20950.022.26596312
TCGTGTA21600.021.49197614
GATCGGA25700.021.2361131
GATCTCG23250.020.43610434
AGCGTCG25450.019.45340210
CGTGTAG24750.019.20028315
GTCGTGT24950.018.43180513
GAGCGTC27500.018.4034149
TCTCGGT26600.017.94507836
AGAGCGT29700.017.262468
GTAGATC28800.017.1096631
ATCGGAA31650.017.0354562
TGGTCGC27950.016.44847342
GTAGGGA30100.016.44444318
GTCGCCG27550.015.73010644
ATCTCGG30350.015.43803135
TGTAGGG36200.013.91677717
GTGTAGG36050.013.73117216
GTGGTCG31800.013.69578241
Produced by FastQC (version 0.11.2)