FastQCFastQC Report
Fri 25 Nov 2016
G146-M31_CAGATC_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M31_CAGATC_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18525810
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC218390.11788418428128108TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA33900.032.117781
TCGTATG34600.030.77542143
ACGTCTG38950.028.57038115
CGTATGC37150.028.54245444
ATCGGAA38650.027.8256442
ACACGTC40650.027.05189313
CGTCTGA40750.026.93066416
CTCGTAT38950.026.77605842
TCTCGTA41950.023.081941
CACGTCT49650.022.50259814
CGGAAGA51700.021.1848134
CACACGT52700.021.0750712
TCGGAAG59400.018.6607883
GCACACG59850.018.59407611
ATCTCGT53500.018.18322840
CATCTCG55700.017.54520239
ACCAGAT69950.016.1372732
AGTCACC74050.015.32680128
GAACTCC79000.014.20039721
CCAGATC80650.013.56230233
Produced by FastQC (version 0.11.2)