FastQCFastQC Report
Fri 25 Nov 2016
G146-M31_CAGATC_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M31_CAGATC_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18525810
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA223550.12066948759595396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT27100.028.33699611
CGTCGTG28450.027.45648412
GATCGGA34000.026.6030221
TCGTGTA29500.025.35770814
GATCTCG31000.024.40980334
GTCGTGT32650.023.52026713
CGTGTAG33500.023.18239815
ATCGGAA39150.022.5978452
AGCGTCG34550.022.29117210
TCTCGGT34550.021.96501536
AGAGCGT37750.021.5093368
GTAGGGA37400.021.11639218
GAGCGTC38400.020.6868159
GTAGATC37450.020.6178231
ATCTCGG37050.020.4239135
GTGGTCG35400.019.448341
TGTAGGG43100.018.5792917
GTGTAGG43750.018.35443116
TGGTCGC39250.018.10171942
AAGAGCG47200.017.3893957
Produced by FastQC (version 0.11.2)