FastQCFastQC Report
Fri 25 Nov 2016
G146-M32_ACTTGA_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M32_ACTTGA_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17606537
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA14500.013.5568641
TCGTATG14300.012.61570543
GTCGGTT18600.011.9934931
GGCGTTA12550.011.74244928
CGTATGC16100.010.65783844
CTCCGAT23450.010.454841
TCGGTTT25500.010.2634882
ATCGGAA18500.010.2238592
ACACGTC19900.09.94655413
CTCCGGT32400.09.8164561
GTCGCAA6150.09.6967431
CTCCGTT28350.09.660641
GCGTTAC15750.09.63634129
CTCGGAT32950.09.5855681
CGTGCGA4153.8562575E-99.5465638
CGTCTGA20100.09.51903316
CTCGAAT29550.09.4178181
TCGGATT18550.09.366372
CCCGGTT18300.09.1727541
CTCCCAT64250.09.14421
Produced by FastQC (version 0.11.2)