FastQCFastQC Report
Fri 25 Nov 2016
G146-M32_ACTTGA_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M32_ACTTGA_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17606537
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT10050.012.91752411
CGTCGTG13500.09.61643312
CGACGAT11000.09.20271
TCGTGTA14200.08.8317714
GATCGGA18500.08.8025831
TTGCGCG6555.456968E-128.3984798
GATCTCG17000.08.28120634
GACGATC18850.08.1722752
TCTCGGT18850.08.05195836
AGCGTCG19050.07.738901610
GTCGTGT17400.07.587570713
CGTGTAG16650.07.53170815
GTAGATC20350.07.45880831
GTCGATT7702.5465852E-117.4307511
CACGATA10850.07.298444742
AATCGTA13850.07.150015
CGACAAT11050.06.97037031
ACGATCC13150.06.8615433
CCGGAAT20400.06.68823671
CGAAGAT24750.06.5797081
Produced by FastQC (version 0.11.2)