FastQCFastQC Report
Fri 25 Nov 2016
G94-M1_TTAGGC.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG94-M1_TTAGGC.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14011493
Sequences flagged as poor quality0
Sequence length67
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26850.012.4966931
TATGCCG27250.012.3117183
CTCGAAT20500.011.4573341
ATGCCGT35550.010.3806434
CCGCAAT9100.010.056031
CGATTAG8250.09.6116494
CGACGAT10750.09.3638011
CTCGGTT24300.09.03801251
CGCAATT7851.6370905E-118.937271
CCGGCAT23600.08.9183351
CCGGGAT28050.08.9171831
CGACAAT10950.08.6356341
CGCCGAT10000.08.5409211
CACGAAT15100.08.4843591
GCCGTCT44100.08.4369866
CGCAGAT26150.08.3986121
CGCCACT30050.08.3236931
TCGAATG15400.08.31812
CCGTCTT44450.08.3019427
CGATCTA6301.7043931E-78.2297544
Produced by FastQC (version 0.11.2)