FastQCFastQC Report
Fri 25 Nov 2016
G94-M2_ACTTGA.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG94-M2_ACTTGA.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19559202
Sequences flagged as poor quality0
Sequence length67
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC41650.014.5742971
TATGCCG41950.014.2498153
TCGTATG30950.011.82509443
ATGCCGT56250.011.3860194
CTCGTAT33750.010.39367642
CGACGAT14950.010.2018361
TCTCGTA37250.010.15415741
AATCTCG35650.010.09655339
CGCAAAT14100.09.9514921
GCCGTCT65150.09.8769836
CGCGAAT7800.09.7767591
CGCATAT7201.8189894E-129.744171
CCGCAAT15300.09.3703531
CCGTCTT67700.09.3247667
CGCCGAT14800.09.2747111
CTCGAAT26800.09.2193381
CCGGAAT30850.09.1955411
CGCCAAT18350.09.14273551
CCGGGAT40150.09.1168591
CGGCAAT22700.09.0032331
Produced by FastQC (version 0.11.2)