FastQCFastQC Report
Fri 25 Nov 2016
G94-M4_TAGCTT.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG94-M4_TAGCTT.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16685541
Sequences flagged as poor quality0
Sequence length67
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC34450.015.1419321
TATGCCG34950.015.096573
ATGCCGT45400.012.4948564
GCCGTCT53600.010.7538036
CCGTCTT57600.010.0599587
TGCCGTC59700.09.7063815
CCCGGAT38350.09.2271381
CTCGAAT24800.08.9793631
GTATGCC58900.08.9580622
CTCCGAT26000.08.9169151
CTCGCAT17450.08.7407551
CGCGCAT7701.0004442E-108.7157821
CGGGAAT28100.08.6847651
CTCGGAT47850.08.6702441
CTCGTTT29450.08.5974011
CCGGGAT33450.08.5724731
CCCGATT15550.08.4355321
CTCCGTT31350.08.3682621
CTCGGTT34900.08.3037171
CGCGCTA5207.6160813E-68.2112252
Produced by FastQC (version 0.11.2)