FastQCFastQC Report
Fri 25 Nov 2016
G94-M5_GGCTAC.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG94-M5_GGCTAC.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21445254
Sequences flagged as poor quality0
Sequence length67
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC48450.012.9072311
TATGCCG51550.012.3064783
ATGCCGT62650.010.3692534
CCGCAAT16550.010.3219811
CGCGAAT7900.010.0396671
CCGGCAT42850.09.7531021
CTCGAAT34000.09.6898691
CGCATAT8950.09.5435091
CGCGGAT12050.09.1135691
GCCGTCT74200.08.9601496
TCGTATG36450.08.953011543
CCGTCTT74750.08.9350217
CCGGAAT34850.08.6657361
CGCAAAT17150.08.5378821
CCGGGAT52700.08.5090241
CGCAATT12200.08.5014321
CGCCGAT13800.08.3999661
ACGGCTA36200.08.34118532
CGCGTAT8802.1827873E-118.3195841
CCCGGAT54500.08.2279921
Produced by FastQC (version 0.11.2)