FastQCFastQC Report
Sat 4 Mar 2017
G101M2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101M2.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences10507470
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC435500.41446704106697424TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA55000.038.5638731
TCGTATG54800.037.8524643
CGTATGC55050.037.60064344
ACACGTC57000.037.2794413
ATCGGAA57900.036.5183532
ACGTCTG58600.036.1861515
CTCGTAT57250.036.15658642
CGTCTGA58800.035.9134316
AATCTCG58450.035.64755239
TCTCGTA60350.034.34019541
CACGTCT62200.034.12714414
CACACGT63000.033.65934412
CGGAAGA64650.033.246734
ATCTCGT65550.031.68541540
TCGGAAG68400.031.102953
GACCAAT67750.031.08061835
GCACACG69200.030.67541311
ACTGACC71950.029.51055132
TGACCAA75850.028.0227434
ACCAATC76550.027.42143436
Produced by FastQC (version 0.11.2)