FastQCFastQC Report
Sat 4 Mar 2017
G122-M10_AGTTCC-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M10_AGTTCC-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18311481
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG11400.013.7118416
GTCGGAT15600.012.9885251
CGTGCGA12100.012.54340938
GGCGTAT9100.011.6170641
TCGGATT19300.011.0650142
GTCGAAA10050.010.9572241
GTCGATT8200.09.9376681
GTCGTTT21500.09.9364191
GTGCGAT15300.09.91995139
CGTCGCG29750.09.8404639
GTCGGTA10200.09.7164791
CGTTCGA16250.09.4838997
GACGTGT14300.09.38175324
GTCGGTT22400.09.3405441
TACTCGT32500.09.2806735
ACGGGTT15550.09.193336524
GCCGGTA15700.09.1182251
GTCCGTT18850.08.7628731
GTCCGAA13700.08.6810011
GTCGGAA18300.08.6651881
Produced by FastQC (version 0.11.2)