FastQCFastQC Report
Sat 4 Mar 2017
G122-M9_AGTCAA-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M9_AGTCAA-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17195852
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA5950.011.46035538
GTCGCAA6300.010.4875421
GTCGGTT23200.010.4423371
ACGTTCG6900.010.210066
GGTACGA13250.09.9692564
TAGTCGG21250.09.84036359
GTCCGGA11650.09.8303811
GTACGAG13600.09.3889385
TCGGTTT26150.09.1762612
GTTACGA7450.09.1607874
TTAGTCG24400.09.1124718
GTCGTTT23800.09.0686391
CGTCGCG11950.09.025579
GTCGGAT11250.09.0053021
TACGAGG15900.08.8615616
CGGTTTG17250.08.6785253
GGACGTC31200.08.60204242
GTCGGTA11650.08.5070611
GGCGTTT17950.08.4659871
AGGACGT31950.08.3997541
Produced by FastQC (version 0.11.2)