FastQCFastQC Report
Sat 4 Mar 2017
G145-M2_CGATGT_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M2_CGATGT_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19326534
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC441340.22835962206156574TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA62750.035.8053131
TCGTATG64050.034.76130743
TATCTCG59850.034.60276839
ATCGGAA66750.033.541532
CTCGTAT66750.032.46557242
ACACGTC71000.032.40084513
TCACCGA68650.032.33333630
CGTATGC68700.032.31239744
ACGTCTG71200.032.06504415
CGTCTGA71500.031.80745916
ACCGATG71250.031.27841232
CCGATGT74750.029.3801633
CACCGAT76050.029.36072231
CGATGTA75100.029.3039634
TCTCGTA71600.028.91802641
GTCACCG78350.028.38651329
CACACGT80350.028.2472212
CACGTCT82000.028.05606314
CGGAAGA81600.027.6261334
ATCTCGT76600.027.32277140
Produced by FastQC (version 0.11.2)